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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Methiocarb

0.0451

0.6

0.0133

50% aq. Ethanol

Human

Full Thickness Skin

Abdomen

2.04

Static

50% aq. Ethanol

Water Bath

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
													9	10

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

NOTES

Prior to the experiment the skin was exposed to the receptor fluid for 18h to ensure that it was fully hydrated. Skin was occluded with parafilm during the experiment to prevent evaporation. Human Cadaver skin was used from white women. 2.04cm2 was the median exposure area. Lag time was determined by extrapolation and is rough.

REFERENCE

Dermal In Vitro Penetration Of Methiocarb, Paclobutrazol and Pirimicarb (2000) Occup. Environ. Med., 57 :734-737