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In Vitro Study No: 1001

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Carbaryl1.280.010.0440% Acetone + 15% DEETPigDermatomed to 0.2-0.3mmDorsal0.32Flow-throughKrebs-Ringer bicarbonate buffer, dextose & BSA37Receptor Fluid882Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
77.53 3.75 4.47 8.66 0.44      

NOTES

Female weanling yorkshire pigs were used. The hair on the dorsal area of the pigs was clipped 48h before harvest. At the end of the experiment the skin was washed with soapy water and the stratum corneum was tape stripped 6 times. Results were given as +/- SE of 4-7 cells so I was unable to convert them to SD for the database.

REFERENCE

The Influence Of Diethyl-m-toluamide (DEET) On The Percutaneous Absorption Of Permethrin And Carbaryl (1997) Toxicol. Appl. Pharmacol., 144 :332-339