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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1003

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Carbaryl

1.28

0.01

0.04

40% DMSO

Pig

Dermatomed to 0.2-0.3mm

Dorsal

0.32

Flow-through

Krebs-Ringer bicarbonate buffer, dextose & BSA

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
82.84		7.76		26.17		36.86		2.94

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

82.84

7.76

26.17

36.86

2.94

NOTES

Female weanling yorkshire pigs were used. The hair on the dorsal area of the pigs was clipped 48h before harvest. At the end of the experiment the skin was washed with soapy water and the stratum corneum was tape stripped 6 times. Results were given as +/- SE of 4-7 cells so I was unable to convert them to SD for the database.

REFERENCE

The Influence Of Diethyl-m-toluamide (DEET) On The Percutaneous Absorption Of Permethrin And Carbaryl (1997) Toxicol. Appl. Pharmacol., 144 :332-339