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In Vitro Study No: 1038

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Methyl-Parathion0.79220.030.0134AcetoneHumanFull Thickness SkinAbdomen1.77StaticSaline, 4% BSA & gentamycin sulphate32Skin48482Gas Chromatography

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
        3.581.780.0105  37

NOTES

Human Cadaver Skin was used. All samples were from the same subject, a 60 year old male with no skin diseases. The loading was given as 51.07nmol/cm2 and the concentration was given as 3.01micromol/ml. I converted these figures from mols to grams and used them together with the volume added to calculate the area of skin exposed. The figure calculated for this does not agree with that calculated for the next study however. They did give kp as 0.00044, but this does not seem to agree with the concentration that they gave so I have left this off the database.

REFERENCE

In Vitro Percutaneous Penetration Of Methyl-Parathion From A Commercial Formulation Through The Human Skin (1997) Occup. Environ. Med., 54 :524-525