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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1040

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Dimethylnitrosamine

0.5

Isopropyl myristate + Sucrose(3H)

Human

Isolated Epidermis

Abdomen

Static

PBS (pH 7.4)

Skin

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
8.39	17.08		1.3	0.78		3.35		2.57	3.45				8

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

8.39

17.08

1.3

0.78

3.35

2.57

3.45

NOTES

Female Abdominal skin was obtained at autopsy. The results were given as +/- SE. I have converted these to SD for the purpose of this database. [3H]Sucrose was added to provide a continuous check of membrane integrity. There was a considerable loss of DMN throughout the experiment. Probably due to evaporation.

REFERENCE

Percutaneous Penetration Of Dimethylnitrosamine Through Human Skin In Vitro: Application From Cosmetic Vehicles (1995) Food Chem.Toxicol., 33 :315-322