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In Vitro Study No: 1041

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
n-Nitrosodiethanolamine1.010.50.5Isopropyl myristate + Sucrose(3H)HumanIsolated EpidermisAbdomen1StaticPBS (pH 7.4)32Skin48482Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
109.9415.61 14.145.88 69.47 23.5916.94.1 0.004185.4

NOTES

Female Abdominal skin was obtained at autopsy. The results were given as +/- SE. I have converted these to SD for the purpose of this database. [3H]Sucrose was added to provide a continuous check of membrane integrity.

REFERENCE

Percutaneous Penetration Of Dimethylnitrosamine Through Human Skin In Vitro: Application From Cosmetic Vehicles (1995) Food Chem.Toxicol., 33 :315-322