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In Vitro Study No: 1042

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Dimethylnitrosamine Not Given0.000105oil-in-water (see notes) + Sucrose(3H)HumanIsolated EpidermisAbdomen1StaticPBS (pH 7.4)32Skin48482Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
7.272.94 0.071.2 5.18 3.980.87   12 

NOTES

Female Abdominal skin was obtained at autopsy. The oil in water vehicle had several constiuents including: Amphisol, stearic acid, cetyl alcohol, dimethicone 200, Cetiol LC, propylene glycol, carbomer 940, water and triethanolamine and Germaden IIE, The results were given as +/- SE. I have converted these to SD for the purpose of this database. [3H]Sucrose was added to provide a continuous check of membrane integrity. There was a considerable loss of DMN throughout the experiment. Probably due to evaporation. The loading was extrapolated back from the results (% Absorption/amount absorbed)

REFERENCE

Percutaneous Penetration Of Dimethylnitrosamine Through Human Skin In Vitro: Application From Cosmetic Vehicles (1995) Food Chem.Toxicol., 33 :315-322