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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1044

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

2-Ethoxyethanol

0.01

1.45

Methanol

Human

Dermatomed to 0.28mm

Breast

0.64

Flow-through

MEM, sodium bicarbonate & gentamycin sulphate

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								7.5	3.33	5.487		5.9E-05	5

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

7.5

3.33

5.487

5.9E-05

NOTES

Skin was obtained from healthy females after breast reductions. Results were given +/- SEM. I have converted these to SD for the purpose of this database. Carbon filters were used to trap evaporated material. The material trapped in the filters was extracted and led to total recoveries of greater that 95%

REFERENCE

Percutaneous Penetration And Metabolism Of 2-Ethoxyethanol (2002) Toxicol. Appl. Pharmacol., 180 :74-82