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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1052

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Naphthalene

1.12

0.03

0.019

Acetone

Monkey

Full Thickness Skin

Abdomen

1.77

Static

Saline, 4% BSA & gentamycin

Skin

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
														2.17

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

2.17

NOTES

The concentration applied was given as 147.5nmol/cm2. I have converted this to mg for the database. They also gave the Kp as 5.12E-3 cm/h, but I have not included this until I check how the Kp was calculated. More than one cell was run for each compound but the actual numbers were not given.

REFERENCE

Prediction Of Percutaneous Absorption From Physicochemical Data: A Model Based On Data Of In Vitro Experiments (42) Ann. Occup. Hyg., 4 :267-276