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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 106

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Hydrocortisone0.50.020.005AcetoneMouseFull Thickness SkinNot Given2Flow-throughoxygenated culture medium Not Given16162Radiolabelled (3H)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
95.680.56      18.15.86   20 

NOTES

they also looked at metabolism (data not included). The data was given as +/- SEM. I have converted this to SD. The flow-through rate of the medium was 4 ml/h. Radioactivity was detectable in the receptor fluid within 15 mins of application. Suggesting that there is little or no lag time.

REFERENCE

Skin Absorption And Cutaneous First Pass Metabolism Of Topical Steroids: In Vitro Studies With Mouse Skin In Organ Culture. (1987) Occup. Environ. Med., 241 :482-487