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In Vitro Study No: 1078

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
DEHP0.890.010.0139AcetoneGuinea pigDermatomed to 0.2mmBack0.64Flow-throughHHBSS, gentamycin, BSA37Receptor Fluid24242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
84.71.9 2.941 4726.11.1   5 

NOTES

Integrity tests were performed with tritiated water prior to the experiment. Concentration applied was given as 35.6nmol/cm2. Extra experimental detail was obtained from ref no 40. They also looked at the percentage of the dose that was metabolised, though this data had not been included. % penetrated is the amount found in the receptor fluid and the amount recovered in the skin discs after washing. n was actually given as >5 cells

REFERENCE

Percutaneous Absorption And Metabolism Of Pyrene, Benzo[a]pyrene, and Di(2-ethylhexyl) Phthalate: Comparison Of In Vitro And In Vivo Results In The Hairless Guinea Pig (1992) Toxicol. Appl. Pharmacol., 115 :216-223