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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 109

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Estriol

0.5

0.02

0.005

Acetone

Mouse

Full Thickness Skin

Not Given

flow-through

oxygenated culture medium

Not Given

Radiolabelled (3H)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
88.66	1.57							2.35	1.27				14

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

88.66

1.57

2.35

1.27

NOTES

they also looked at metabolism (data not included). The flow-through rate of the medium was 4 ml/h. The data was given as +/- SEM. I have converted this to SD. Radioactivity was detectable in the receptor fluid within 15 mins of application. Suggesting that there is little or no lag time.

REFERENCE

Skin Absorption And Cutaneous First Pass Metabolism Of Topical Steroids: In Vitro Studies With Mouse Skin In Organ Culture. (1987) Occup. Environ. Med., 241 :482-487