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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1099

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

2(2-Methoxyethoxy)ethanol

0.644

0.263

JP-8

Rat

Dermatomed to 0.56mm

Back

4.9

Static

6% Volpo20 in PBS

Skin

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										51.5	15.1	0.08	10

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

51.5

15.1

0.08

NOTES

Male Fischer-344 rats were used. The cell was occluded with a glass stopper. The flux for this compund was calculted from the slope of the first 2 hoursbecause the concentration at the surface diminished during the experiment due to the rapid penetration.

REFERENCE

Assessment Of Skin Absorption And Penetration Of JP-8 Jet Fuel And Its Components (2000) Toxicol. Sci., 55 :247-255