IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 110
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Triclosan
18.68
0.007
0.204
90% aq. Ethanol
Rat
Dermatomed to 0.28mm
Dorsal
0.64
flow-through
Eagles MEM & Earles salts buffered to pH7.3
32
Receptor Fluid
24
24
2
Radiolabelled (3H)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
99
25.1
5.6
18.2
5.9
66.1
22.8
15.9
25422.49
12
7
NOTES
There appears to have been an error in the calculation of the flux. Receptor Fluid Pumped through at 1.5ml per hour. The results were given as +/- SEM. I have converted this to SD.
REFERENCE
Percutaneous Penetration And Dermal Metabolism Of Triclosan (2000) J. Pharmacol. Exp. Ther., 38 :361-370
