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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1101

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Isofenphos

2.64

0.005

0.0132

Acetone

Human

Dermatomed to 0.5mm

Thigh

Flow-through

Human plasma

Water Bath

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
88.7	4.6		2.4	6.5		9		2.5	2				4

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

88.7

4.6

2.4

6.5

2.5

NOTES

Human thigh skin was obtained at autopsy from a 16 year old white male. The top section of the cell had an extended cylinder wall that inhibited normal air flow across the skin.

REFERENCE

In Vivo And In Vitro Percutaneous Absorption And Skin Evaporation Of Isofenphos In Man (1992) Fundam. Appl. Toxicol., 19 :521-526