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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 1105

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Lidocaine33.710.7539.540% propylene glycol & NaOH (pH7.8)MouseFull Thickness SkinDorsal/Ventral0.64Flow-throughSalijne & 0.25% chlorobutanol37Receptor Fluid  1Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          616103 31.03

NOTES

Female hairless mice were used. The donor chamber was sealed. More experimental detail can be obtained from Kushla GP and Zatz JL. J. Soc. Cosm. Chem. 40 (1989) 41-50. Flow rate was 1.5ml/h

REFERENCE

Influence Of pH On Lidocaine Penetration Through Human And Hairless Mouse Skin In Vitro (1991) Int. J. Pharm., 71 :167-173