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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1106

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Lidocaine

19.58

0.75

22.95

40% propylene glycol & NaOH (pH10)

Mouse

Full Thickness Skin

Dorsal/Ventral

0.64

Flow-through

Salijne & 0.25% chlorobutanol

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										511	106		3	0.74

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

511

106

0.74

NOTES

Female hairless mice were used. The donor chamber was sealed. More experimental detail can be obtained from Kushla GP and Zatz JL. J. Soc. Cosm. Chem. 40 (1989) 41-50. Flow rate was 1.5ml/h

REFERENCE

Influence Of pH On Lidocaine Penetration Through Human And Hairless Mouse Skin In Vitro (1991) Int. J. Pharm., 71 :167-173