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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 1107

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Lidocaine660.7577.640% propylene glycol & HCl (pH4)HumanDermatomed to 0.15mmLeg0.64Flow-throughSalijne & 0.25% chlorobutanol37Receptor Fluid  1Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          22.75.1 31.31

NOTES

The skin came from the amputated leg of a 70 year old woman. The donor chamber was sealed. The solubiliity was found to be 437.3 mg/ml The concentration added was given as around 5% w/w. I have worked out the concentration in mg/ml from the results as estimated flux from a saturated solution was given as well as the experimental flux achieved. More experimental detail can be obtained from Kushla GP and Zatz JL. J. Soc. Cosm. Chem. 40 (1989) 41-50. Flow rate was 1.5ml/h

REFERENCE

Influence Of pH On Lidocaine Penetration Through Human And Hairless Mouse Skin In Vitro (1991) Int. J. Pharm., 71 :167-173