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In Vitro Study No: 111

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Triclosan18.680.0070.20490% aq. EthanolHumanDermatomed to 0.23mmBreast and Abdomen0.64Flow-throughEagles MEM & Earles salts buffered to pH7.332Receptor Fluid24242Radiolabelled (3H)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
98.1 21.86.323.74.751.8 6.33.483619.375  103

NOTES

There appears to have been an error in the calculation of the flux. Receptor Fluid Pumped through at 1.5ml per hour. The results were given as +/- SEM. I have converted this to SD.

REFERENCE

Percutaneous Penetration And Dermal Metabolism Of Triclosan (2000) J. Pharmacol. Exp. Ther., 38 :361-370