IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1110
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Lidocaine
19.58
0.75
22.95
40% propylene glycol & NaOH (pH10)
Human
Dermatomed to 0.15mm
Leg
0.64
Flow-through
Salijne & 0.25% chlorobutanol
37
Receptor Fluid
1
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
208
29
3
0.89
NOTES
The skin came from the amputated leg of a 70 year old woman. The donor chamber was sealed. More experimental detail can be obtained from Kushla GP and Zatz JL. J. Soc. Cosm. Chem. 40 (1989) 41-50. Flow rate was 1.5ml/h
REFERENCE
Influence Of pH On Lidocaine Penetration Through Human And Hairless Mouse Skin In Vitro (1991) Int. J. Pharm., 71 :167-173