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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1140

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Corticosterone

0.296

Not Given

pH 7.2 phosphate buffer

Snake

Full Thickness Skin

Not Given

Static

Isotonic buffer pH 7.2

Water Bath

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.000127

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.000127

NOTES

Shed snake skin of Elaphe obsoleta (black rat snake) were used. More experimental detail is available from: M. Akazawa, T. Itoh, K. Masaki, BT Nghiem et al Int. J. Pharm. 50:53-60 (1989). A saturated solution was used. N was between 3 and 4.Permeabilities were calculated from the straight portion of the graph 24-72h. Lag time was between 6-8 h

REFERENCE

A Method To Predict The Percutaneous Permeability Of Various Compunds: Shed Snake Skin As A Model Membrane (1990) Pharmaceut. Res., 7 :1302-1306