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In Vitro Study No: 1143

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Naproxen0.0361Not Given pH 3 phosphate bufferSnakeFull Thickness SkinNot Given StaticIsotonic buffer pH 7.237Water Bath24242HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
            0.0148  

NOTES

Shed snake skin of Elaphe obsoleta (black rat snake) were used. More experimental detail is available from: M. Akazawa, T. Itoh, K. Masaki, BT Nghiem et al Int. J. Pharm. 50:53-60 (1989). The donor solution was saturated. N was between 4 and 5. The lag time was short - between 1 and 3 hours

REFERENCE

A Method To Predict The Percutaneous Permeability Of Various Compunds: Shed Snake Skin As A Model Membrane (1990) Pharmaceut. Res., 7 :1302-1306