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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1146

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

11a-hydroxyprogesterone

0.176

Not Given

pH 7.2 phosphate buffer

Snake

Full Thickness Skin

Not Given

Static

Isotonic buffer pH 7.2

Water Bath

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.000826

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.000826

NOTES

Shed snake skin of Elaphe obsoleta (black rat snake) were used. More experimental detail is available from: M. Akazawa, T. Itoh, K. Masaki, BT Nghiem et al Int. J. Pharm. 50:53-60 (1989). A saturated solution of 11a-HPG was added. N was between 3 and 4. Permeabilities were calculated from the straight portion of the graph 24-72h. Lag time was between 6-8h

REFERENCE

A Method To Predict The Percutaneous Permeability Of Various Compunds: Shed Snake Skin As A Model Membrane (1990) Pharmaceut. Res., 7 :1302-1306