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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1173

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Phenol

Water

Human

Stratum Corneum Membrane

Abdomen

Static

Water

Not Given

UV spectrometry

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										1.49			33	0.55

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

1.49

0.55

NOTES

Skin was separated by the heat separation method of Kligman and Christophers (1963) Omitting the trypsinisation step so as to avoid damaging the membrane. A stainless steel mesh was used to support the membrane. The effect of the mesh and its diffusion layers was said to be negligable in comparison to the skin (data not given). Skin came from 10 different donors.

REFERENCE

Variations In Permeability Of Human Skin Within And Between Specimens (1984) Int. J. Pharm., 18 :299-309