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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 1177

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Phenol101 WaterHumanStratum Corneum MembraneAbdomen StaticWater22Not Given10102UV spectrometry

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          1.38  20.4

NOTES

Skin was separated by the heat separation method of Kligman and Christophers (1963) Omitting the trypsinisation step so as to avoid damaging the membrane. A stainless steel mesh was used to support the membrane. The effect of the mesh and its diffusion layers was said to be negligable in comparison to the skin (data not given). Skin came from a single elderly caucasian donor.

REFERENCE

Variations In Permeability Of Human Skin Within And Between Specimens (1984) Int. J. Pharm., 18 :299-309