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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1193

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Dinitrochlorobenzene

Not Given

10% Dimethylformamide

Human

Isolated Epidermis

Not Given

Static

Not Given

Gas chromatography

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										286.1	52.5		6

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

286.1

52.5

NOTES

Epidermal membranes were prepared by heat separation. An integrity test was done with tritiated water. There is no indication that steady-state was actually reached. Figure should just be taken as average absorption rate.

REFERENCE

In Vitro Absorption Of 1-chloro 2,4-dinitrobenzene (DNCB) Through Human, Hooded Rat And Mouse Epidermis (1986) Br. J. Dermatol., 115 :47-48