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In Vitro Study No: 1197

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Dinitrochlorobenzene Not Given 10% DimethylformamideRatIsolated EpidermisNot Given StaticNot Given Not Given  2Gas chromatography

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          1161.1227.5 7 

NOTES

Epidermal membranes were prepared by soaking in NaBr solution. An integrity test was done with tritiated water. There is no indication that steady-state was actually reached. Figure should just be taken as average absorption rate.

REFERENCE

In Vitro Absorption Of 1-chloro 2,4-dinitrobenzene (DNCB) Through Human, Hooded Rat And Mouse Epidermis (1986) Br. J. Dermatol., 115 :47-48