IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1198
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Dinitrochlorobenzene
Not Given
10% Dimethylformamide
Mouse
Isolated Epidermis
Not Given
Static
Not Given
Not Given
2
Gas chromatography
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
1736.7
256.6
5
NOTES
Epidermal membranes were prepared by soaking in NaBr solution. An integrity test was done with tritiated water. . There is no indication that steady-state was actually reached. Figure should just be taken as average absorption rate.
REFERENCE
In Vitro Absorption Of 1-chloro 2,4-dinitrobenzene (DNCB) Through Human, Hooded Rat And Mouse Epidermis (1986) Br. J. Dermatol., 115 :47-48
