IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1200
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Benzoic Acid
See Notes.
0.004
Petrolatum
Rat
Full Thickness Skin
Back
1.13
Static
Saline & 0.055 Thimerosal
32
Water Bath
5
5
2
Radioolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.5
0.2
10
NOTES
Male Osborne-Mendel Rats were used. 30mg of petrolatum was added. The concentration added was 160ng/mg
REFERENCE
Differences In Permeability Of Rat Skin Related To Sex And Body Site (1983) J. Soc. Cosmet. Chem., 34 :127-135
