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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1210

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Urea

0.000854

0.3

0.00023

Water

Rat

Full Thickness Skin

Back

1.13

Static

Saline & 0.055 Thimerosal

Water Bath

Radioolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.00016	6	15

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.00016

NOTES

Male Osborne-Mendel Rats were used. The concentration of urea added was given as 854ng/mg. The top of the chamber was sealed with Parafilm to prevent evaporation of the vehicle.

REFERENCE

Differences In Permeability Of Rat Skin Related To Sex And Body Site (1983) J. Soc. Cosmet. Chem., 34 :127-135