IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1211
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Urea
0.000854
0.3
0.00023
Water
Rat
Full Thickness Skin
Abdomen
1.13
Static
Saline & 0.055 Thimerosal
32
Water Bath
2
Radioolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.00188
4
16.5
NOTES
Female Osborne-Mendel Rats were used. The concentration of urea added was given as 854ng/mg. The top of the chamber was sealed with Parafilm to prevent evaporation of the vehicl
REFERENCE
Differences In Permeability Of Rat Skin Related To Sex And Body Site (1983) J. Soc. Cosmet. Chem., 34 :127-135