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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1227

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Hydrocortisone

1.4

Saline

Mouse

Full Thickness Skin

Abdomen

0.6

Static

Saline

Water Bath

Radiolabelled (3H)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.0001	3	7.6

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.0001

7.6

NOTES

5 day old. Male hairless mice of SKH-hr-1 strain were used. The concentration of Hydrocortisone added was less than 1E-5M. There was a biphasic absorption with Kp increasing to 4.1E-4cm/h after around 30 hours. They calculated the lag time for this phase to be 21.5h.

REFERENCE

Percutaneous Absorption Of Corticosteroids: Age, Site and Skin-Sectioning Influences On Rates Of Permeation Of Hairless Mouse Skin By Hydrocortisone (1984) J. Pharm. Sci., 73 :1287-1290