IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1228
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Hydrocortisone
1.4
Saline
Mouse
Full Thickness Skin
Dorsal
0.6
Static
Saline
37
Water Bath
40
40
2
Radiolabelled (3H)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.00012
3
9.9
NOTES
5 day old. Male hairless mice of SKH-hr-1 strain were used. The concentration of Hydrocortisone added was less than 1E-5M. There was a biphasic absorption with Kp increasing to 2.4E-4 cm/h they calculated the lag time for this phase to be 17.8h.
REFERENCE
Percutaneous Absorption Of Corticosteroids: Age, Site and Skin-Sectioning Influences On Rates Of Permeation Of Hairless Mouse Skin By Hydrocortisone (1984) J. Pharm. Sci., 73 :1287-1290