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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1239

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Chlorpyrifos

18.283

0.02

0.731

Commercial solution containing xylene & 111-TCE

Human

Full Thickness Skin

Breast

0.5

Flow-through

50% aq. Ethanol

Skin

Gas Chromatography

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
95				15		34.7		19.7				0.00025	10

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

34.7

19.7

0.00025

NOTES

5 minutes was allowed for solvent to evaporate and then the top of the cell was occluded with parafilm. 2 different donors were used. The concentration of Chlorpyrifos added was given as 52 150 nmoles/ml. Propetamphos was used as an internal standard during the analysis.

REFERENCE

The In Vitro Percutaneous Penetration Of Chlorpyrifos (2000) Hum. Exp. Toxicol., 19 :104-107