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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 1244

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Trichloromethane0.90.050.0703Distilled WaterHumanDermatomed to 0.3mmAbdomen0.64Flow-throughNot Given Not Given442Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
95.789.47 0.0630.14 7.141.3771.32  0.016  

NOTES

Fresh abdominal skin was obtained from female patients undergoing paniculectomy operations. Immediately after dosing, scrwe-cap lids which iincluded a wire mesh and gauze basket containing 14-60 mesh activated charcoal were placed tightly on the flow-through cells. They do not mention what the receptor fluid was, how many studies were done or the temperature that they were conducted at. Most of the radioactivity was recovered from the charcoal trap (76.6%)

REFERENCE

In Vitro And In Vivo Percutaneous Absorption Of 14C-Chloroform In Humans (1995) Hum. Exp. Toxicol., 14 :260-265