IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1245
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Theophylline
Not Given
PEG 400
Rat
Dermatomed to 0.45mm
Dorsal
0.64
Flow-through
Normal saline & 0.25% w/v chlorobutanol
37
Receptor Fluid
24
24
2
Reversed phase HPLC with UV detection
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
1.98
9
NOTES
Male fuzzy rat (Skh:fz) skin was used. A saturated solution of Theophylline in PEG 400 was added.
REFERENCE
The Effect Of Solvents On Solute Penetration Through Fuzzy Rat Skin In Vitro (1989) J. Soc. Cosmet. Chem., 40 :231-242
