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In Vitro Study No: 1248

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Theophylline Not Given Propylene glycolRatDermatomed to 0.45mmDorsal0.64Flow-throughNormal saline & 0.25% w/v chlorobutanol37Receptor Fluid24242Reversed phase HPLC with UV detection

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          7.93  6 

NOTES

Male fuzzy rat (Skh:fz) skin was used. A saturated solution of Theophylline in propylene glycol was added.

REFERENCE

The Effect Of Solvents On Solute Penetration Through Fuzzy Rat Skin In Vitro (1989) J. Soc. Cosmet. Chem., 40 :231-242