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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1252

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Theophylline

Not Given

Water

Rat

Dermatomed to 0.45mm

Dorsal

0.64

Flow-through

Normal saline & 0.25% w/v chlorobutanol

Receptor Fluid

Reversed phase HPLC with UV detection

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										21.62	2.6		6

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

21.62

2.6

NOTES

Male fuzzy rat (Skh:fz) skin was used. A saturated aqueous solution was used. 6 cells using skin from the same animal were run.

REFERENCE

The Effect Of Solvents On Solute Penetration Through Fuzzy Rat Skin In Vitro (1989) J. Soc. Cosmet. Chem., 40 :231-242