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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 126

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Morphine

1.67

isotonic phosphate buffer (pH7.4)

Hairless Mouse

Full Thickness Skin

Dorsal/Abdomen

1.2

Static

isotonic phosphate buffer & sodium azide

Receptor Fluid

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										0.3	0.1	0.00015	3	0.08

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.3

0.1

0.00015

0.08

NOTES

The steady state flux was calculated by linear regression analysis of permeation data. It was given +/- SE, I have converted this to SD for this database. I have calculated the permeability coefficient from the steady state flux divided by the concentration. Some details were extracted form Monti et al (1995) J. Cont. Release, 33 71-77

REFERENCE

Comparison Of The Effect Of Ultrasound And Of Chemical Enhancers On Transdermal Permeation Of Caffeine And Morphine Through Hairless Mouse Skin In Vitro (2001) Int. J. Pharm., 229 :131-137