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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1262

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Methyl-4-hydroxybenzoate

470

Not Given

Methanol

Human

Dermatomed to 0.45mm

Dorsal

0.64

Flow-through

Normal saline & 0.25% w/v chlorobutanol

Receptor Fluid

Reversed phase HPLC with UV detection

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										1384.47			6

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

1384.47

NOTES

Male fuzzy rat (Skh:fz) skin was used. A saturated solution of methyl paraben in Methanol was used.

REFERENCE

The Effect Of Solvents On Solute Penetration Through Fuzzy Rat Skin In Vitro (1989) J. Soc. Cosmet. Chem., 40 :231-242