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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1267

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

2-phenylphenol

1.536

0.05

0.12

60% aq. Ethanol

Human

Full Thickness Skin

Abdomen

0.64

Static

Dulbecco'sMEM, HEPES: Ham F-12, glutamx-I, BSA, L-glutamine, hydrocortisone & gentamycin

Incubator

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
						91.92	1.25	0.75	0.42				5	3.8

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

91.92

1.25

0.75

0.42

3.8

NOTES

human skin was obtained after abdominal surgery from young caucasian females. After 4h the remaining test compound was removed from the application site with cotton swabs. The experiment was conducted 4-6 times. I have used the median of this in the database. The results were given as mcrg/cm2. I have converted these into % for this database. The % penetrated was worked out as the amount applied minus the amount not absorbed after a 4h exposure.

REFERENCE

Comparative In Vitro-In Vivo Percutaneous Penetration Of The Fungicide Ortho-Phenylphenol (2002) Regul. Toxicol. Pharmacol., 35 :198-208