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In Vitro Study No: 127

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Diazinon Not Given0.00025AcetoneHumanDermatomed to 0.5mmUnknown1Flow-throughBuffered Saline Not Given24242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
60.76.6 1.75.2 19.3 14.19.2   12 

NOTES

6 samples from 2 volunteers were used. Only 60.7% of the applied dose was recovered in total. The Potential Absorption has been calculated from the amount found in the receptor fluid (14.1%) + the amount recovered from the skin digest (5.2%). 41.4% was recovered in the surface wash. Compound is highly lipophillic and the receptor fluid is aqueous which may have affected the results.

REFERENCE

Percutaneous Absorption Of Diazinon In Humans (1993) Food Chem.Toxicol., 8 :569-572