IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1270
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
2-phenylphenol
1.536
0.05
0.12
60% aq. Ethanol
Human
Full Thickness Skin
Abdomen
0.64
Static
Dulbecco'sMEM, HEPES: Ham F-12, glutamx-I, BSA, L-glutamine, hydrocortisone & gentamycin
32
Incubator
4
48
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
91.92
1.25
31.92
4.75
0.00159
5
3.8
NOTES
human skin was obtained after abdominal surgery from young caucasian females. After 4h the remaining test compound was removed from the application site with cotton swabs. However, the receptor fluid was collected for 48h. The experiment was conducted 4-6 times. I have used the median of this in the database. The results were given as mcrg/cm2. I have converted these into % for this database. The s-s flux was not calculated but the maximal flux was 1.11 mcrg/cm2/h. The % penetrated was worked out as the amount applied minus the amount not absorbed after a 4h exposure.
REFERENCE
Comparative In Vitro-In Vivo Percutaneous Penetration Of The Fungicide Ortho-Phenylphenol (2002) Regul. Toxicol. Pharmacol., 35 :198-208
