EDETOX logo

IN VITRO STUDIES FURTHER DETAILS


BACK TO THE MAIN MENU

back to previous page forward to next page

University of Newcastle homepage

In Vitro Study No: 1273

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
2-phenylphenol1.5360.050.1260% aq. EthanolHumanIsolated EpidermisAbdomen0.64StaticSaline (0.9% NaCl), 0.01% sodium azide & 3% BSA32Incubator4242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
      91.921.3385.926.25   50.2

NOTES

human skin was obtained after abdominal surgery from young caucasian females. After 4h the remaining test compound was removed from the application site with cotton swabs. However, the receptor fluid was collected for 24h. The experiment was conducted 4-6 times. I have used the median of this in the database. The results were given as mcrg/cm2. I have converted these into % for this database. The % penetrated was worked out as the amount applied minus the amount not absorbed after a 4h exposure.

REFERENCE

Comparative In Vitro-In Vivo Percutaneous Penetration Of The Fungicide Ortho-Phenylphenol (2002) Regul. Toxicol. Pharmacol., 35 :198-208