IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 129
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Testosterone
1.154
0.01
0.0577
95% aq. Ethanol
Human
Dermatomed to 0.6mm
Breast/Abdomen
0.2
Static
isotonic saline % 1.5% BSA
37
Receptor Fluid
8
8
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
1.86
5.58
6.91
1.33
0.54
6
NOTES
They added 0.2microCi/microlitre and 4nmol/microlitre. Specific Activity was 81Ci/mmol. Data was given +/- SEM. I have converted this to SD for the database.
REFERENCE
Role Of Transepidermal And Transfollicular Routes In Percutaneous Absorption Of Steriods: In Vitro Studies On Human Skin (1994) Skin Pharmacol., 7 :237-244