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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 1293

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Benzoic Acid20.010.01AcetoneMouseFull Thickness SkinNot Given2Flow-throughMEM Not Given662Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
78.61.6      75.52.3   3 

NOTES

Male HRS/J hairless mice were used. The skin was fresh and placed into the chamber within an hour of excision. The % absorption was calculated by adding together the amounts recovered in the receptor fluid, the skin support and the lower chamber of the flow-through cell. A flow rate of 2.5ml/h was used. This experiment was run twice in an attempt to show reproducibility. The second run is in vitro study 1289

REFERENCE

Evaluation Of A Flow Through In Vitro Skin Penetration Chamber Method Using Acetone-Deposited Organic Solids (1990) J. Toxicol.:Cutaneous Ocul. Toxicol., 9 :277-299