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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1297

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Acetylsalicylic Acid

0.01

Acetone

Rat

Full Thickness Skin

Not Given

Flow-through

MEM

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
95.1	3							6.8	1.7				3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

95.1

6.8

1.7

NOTES

Male Fischer 344 Rats were used. The skin was fresh and placed into the chamber within an hour of excision. The % absorption was calculated by adding together the amounts recovered in the receptor fluid, the skin support and the lower chamber of the flow-through cell. A flow rate of 4.2ml/h was used.

REFERENCE

Evaluation Of A Flow Through In Vitro Skin Penetration Chamber Method Using Acetone-Deposited Organic Solids (1990) J. Toxicol.:Cutaneous Ocul. Toxicol., 9 :277-299