EDETOX logo

IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1320

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Sodium Laurate

1.3

0.25

0.0663

Water

Rat

Full Thickness Skin

Dorsal

4.9

Static

Saline

Incubator

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								1.51	0.45				3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

1.51

0.45

NOTES

Female Colworth Wistar Rats were used. The soap solution was made by adding dodecanoic acid into a Duall glass homogeniser with excess diethyl ether which was removed un a stream of nitrogen. Dilute NaOH was added, the resulting solution was homogenised and equilabrated for 245h at 40oC before adjusting the pH to 9.5

REFERENCE

The Percutaneous Absorption Of Some Anionic Surfactants (1975) J. Soc. Cosmet. Chem., 26 :47-63