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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1325

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Sodium Palmitate

1.7

0.25

0.0867

Water

Rat

Full Thickness Skin

Dorsal

4.9

Static

Saline

Incubator

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								0.23	0.12				3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.23

0.12

NOTES

Female Colworth Wistar Rats were used. The soap solution was made by adding hexadecanoic acid into a Duall glass homogeniser with excess diethyl ether which was removed un a stream of nitrogen. Dilute NaOH was added, the resulting solution was homogenised and equilibrated for 245h at 40oC before adjusting the pH to 9.5

REFERENCE

The Percutaneous Absorption Of Some Anionic Surfactants (1975) J. Soc. Cosmet. Chem., 26 :47-63