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In Vitro Study No: 1329

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Sodium Laurate1.30.10.1667WaterHumanIsolated EpidermisAbdomen0.78StaticSaline, 0.012% Penicillin & 0.01% Streptomycin.37Incubator222Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
        0.240.12   4 

NOTES

Female abdominal skin samples were obtained at autopsy. The soap solution was made by adding dodecanoic acid into a Duall glass homogeniser with excess diethyl ether which was removed un a stream of nitrogen. Dilute NaOH was added, the resulting solution was homogenised and equilibrated for 245h at 40oC before adjusting the pH to 9.5

REFERENCE

The Percutaneous Absorption Of Some Anionic Surfactants (1975) J. Soc. Cosmet. Chem., 26 :47-63